talon metal affinity resin column (TaKaRa)
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TaKaRa
talon metal affinity resin column
Talon Metal Affinity Resin Column, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 5144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/talon+metal+affinity+column/TALON+Metal+Affinity+Resin/pm41839924-245-6-11
Average 99 stars, based on 5144 article reviews
Talon Metal Affinity Resin Column, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 5144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/talon+metal+affinity+column/TALON+Metal+Affinity+Resin/pm41839924-245-6-11
Average 99 stars, based on 5144 article reviews
talon metal affinity resin column - by Bioz Stars,
2026-09
99/100 stars
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Recombinant:Article Title: Engineering stable and non-immunogenic immunoenzymes for cancer therapy via in situ generated prodrugs. Article Snippet: Engineering human enzymes for therapeutic applications is attractive but introducing new amino acids may adversely affect enzyme stability and immunogenicity.. Here we used a mammalian membrane-tethered screening system (ECSTASY) to evolve human lysosomal beta-glucuronidase (hBG) to hydrolyze a glucuronide metabolite (SN-38G) of the anticancer drug irinotecan (CPT-11).. Three human beta-glucuronidase variants (hBG3, hBG10 and hBG19) with 3, 10 and 19 amino acid substitutions were identified that display up to 40-fold enhanced enzymatic activity, higher stability than E. coli beta-glucuronidase in human serum, and similar pharmacokinetics in mice as wild-type hBG. Purification:Article Title: Engineering stable and non-immunogenic immunoenzymes for cancer therapy via in situ generated prodrugs. Article Snippet: Engineering human enzymes for therapeutic applications is attractive but introducing new amino acids may adversely affect enzyme stability and immunogenicity.. Here we used a mammalian membrane-tethered screening system (ECSTASY) to evolve human lysosomal beta-glucuronidase (hBG) to hydrolyze a glucuronide metabolite (SN-38G) of the anticancer drug irinotecan (CPT-11).. Three human beta-glucuronidase variants (hBG3, hBG10 and hBG19) with 3, 10 and 19 amino acid substitutions were identified that display up to 40-fold enhanced enzymatic activity, higher stability than E. coli beta-glucuronidase in human serum, and similar pharmacokinetics in mice as wild-type hBG. Article Title: Irisin acts through its integrin receptor in a two-step process involving extracellular Hsp90α Article Snippet: .. Protein mix was purified through Affinity Column:Article Title: Engineering stable and non-immunogenic immunoenzymes for cancer therapy via in situ generated prodrugs. Article Snippet: Engineering human enzymes for therapeutic applications is attractive but introducing new amino acids may adversely affect enzyme stability and immunogenicity.. Here we used a mammalian membrane-tethered screening system (ECSTASY) to evolve human lysosomal beta-glucuronidase (hBG) to hydrolyze a glucuronide metabolite (SN-38G) of the anticancer drug irinotecan (CPT-11).. Three human beta-glucuronidase variants (hBG3, hBG10 and hBG19) with 3, 10 and 19 amino acid substitutions were identified that display up to 40-fold enhanced enzymatic activity, higher stability than E. coli beta-glucuronidase in human serum, and similar pharmacokinetics in mice as wild-type hBG. Article Title: Dendritic cells pulsed with penetratin-OLFM4 inhibit the growth and metastasis of melanoma in mice. Article Snippet: Cancer stem cells (CSCs) can self-renew and differentiate, contributing to tumor heterogeneity, metastasis, and recurrence.. Their resistance to therapies, including immunotherapy, underscores the importance of targeting them for complete remission and relapse prevention.. Olfactomedin 4 (OLFM4), a marker associated with various cancers such as colorectal cancer, is expressed on CSCs promoting immune evasion and tumorigenesis. Article Title: Pif1 Helicase Mediates Remodeling of Protein-Nucleic Acid Complexes by Promoting Dissociation of Sub1 from G-Quadruplex DNA and Cdc13 from G-Rich Single-Stranded DNA. Article Snippet: Pif1 is a molecular motor enzyme that is conserved from yeast to mammals.. It translocates on ssDNA with a directional bias (5′ → 3′) and unwinds duplexes using the energy obtained from ATP hydrolysis.. Pif1 is involved in dsDNA break repair, resolution of G-quadruplex (G4) structures, negative regulation of telomeres, and Okazaki fragment maturation. Article Title: Identification of a Lacosamide Binding Protein Using an Affinity Bait and Chemical Reporter Strategy: 14-3-3 ζ Article Snippet: .. The supernatant was loaded onto a Article Title: Irisin acts through its integrin receptor in a two-step process involving extracellular Hsp90α Article Snippet: .. Protein mix was purified through Article Title: A three-step "ping-pong" mechanism of a GH20 β-N-acetylglucosaminidase from Vibrio campbellii belonging to a major Clade A-I of the phylogenetic tree of the enzyme superfamily. Article Snippet: β-N-acetylglucosaminidase (GlcNAcase) is an essential biocatalyst in chitin assimilation by marine Vibrio species, which rely on chitin as their main carbon source.. Structure-based phylogenetic analysis of the GlcNAcase superfamily revealed that a GlcNAcase from Vibrio campbellii, formerly named V. harveyi, (VhGlcNAcase) belongs to a major clade, Clade A-I, of the phylogenetic tree.. Pre-steady-state and steady-state kinetic analysis of the reaction catalysed by VhGlcNAcase with the fluorogenic substrate 4-methylumbelliferyl N-acetyl-β-D-glucosaminide suggested the following mechanism: (1) the Michaelis-Menten complex is formed in a rapid enzymesubstrate equilibrium with a Kd of 99.1 ± 1 μM. (2) The glycosidic bond is cleaved by the action of the catalytic residue Glu438, followed by the rapid release of the aglycone product with a rate constant (k2) of 53.3 ± 1 s 1. (3) After the formation of an oxazolinium ion intermediate with the assistance of Asp437, the anomeric carbon of the transition state is attacked by a catalytic water, followed by release of the glycone product with a rate constant (k3) of 14.6 s 1, which is rate-limiting. Expressing:Article Title: Pif1 Helicase Mediates Remodeling of Protein-Nucleic Acid Complexes by Promoting Dissociation of Sub1 from G-Quadruplex DNA and Cdc13 from G-Rich Single-Stranded DNA. Article Snippet: Pif1 is a molecular motor enzyme that is conserved from yeast to mammals.. It translocates on ssDNA with a directional bias (5′ → 3′) and unwinds duplexes using the energy obtained from ATP hydrolysis.. Pif1 is involved in dsDNA break repair, resolution of G-quadruplex (G4) structures, negative regulation of telomeres, and Okazaki fragment maturation. |